G. Caimi et al., Polymorphonuclear leucocyte rheology and cytosolic Ca2+ content after activation in chronic renal failure, NEPHROLOGY, 6(3), 2001, pp. 113-117
We evaluated polymorphonuclear leucocyte (PMN) flow properties in patients
with clinically stable chronic renal failure (CRF) and in control subjects
at baseline and after activation with 4-phorbol 12-myristate 13-acetate (PM
A) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). Initial relative flo
w rate (IRFR) and clogging particles (CPs) were obtained using the St. Geor
ge's Filtrometer, and PMN membrane fluidity was assessed by marking PMNs wi
th 1-(4-(trimethylamino)phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH). PMN cy
tosolic Ca2+ concentration was determined by marking PMNs with Fura 2-AM. A
t baseline, CRF patients showed a significant increase only in PMN cytosoli
c Ca2+ content. After activation with PMA and fMLP, a decrease in IRFR and
an increase in CP were observed in both control subjects and CRF patients,
although the variation in IRFR present in the group of CRF patients was gre
ater than in the control group. After activation with PMA and fMLP, we foun
d a decrease in PMN membrane fluidity only in CRF patients, but no variatio
n in PMN cytosolic Ca2+ concentration in either group was observed. These r
esults provide evidence for PMN dysfunction in chronic renal failure.