Induction of immune responses and safety profiles in rhesus macaques immunized with a DNA vaccine expressing human prostate specific antigen

Prostate specific antigen (PSA) is a widely used marker for prostate cancer , which is secreted by normal prostate cells at low levels, but is produced more substantially by cancer cells. We have previously reported on the use of a DNA vaccine construct that encodes for human PSA gene to elicit host immune responses against cells producing PSA. DNA immunization strategy del ivers DNA constructs encoding for a specific immunogen into the host, who b ecomes the in vivo protein source for the production of antigen. This antig en then is the focus of the resulting immune response. In this study, we ex amine the induction of immune responses and safety profiles in rhesus macaq ues immunized with DNA-based PSA vaccine. We observed induction of PSA-spec ific humoral response as well as positive PSA-specific lymphoproliferative (LPA) response in the vaccinated macaques. We also observed that the stimul ated T cells from the PSA-immunized rhesus macaques produced higher levels of Th1 type cytokine IFN-gamma than the control vector immunized animals. O n the other hand, DNA immunization did not result in any adverse effects in the immunized macaques, as indicated by complete blood counts, leukocyte d ifferentials and hepatic and renal chemistries. The macaques appeared healt hy, without any physical signs of toxicity throughout the observation perio d. In addition, we did not observe any adverse effect on the vaccination si te. The apparent safety and immunogenecity of DNA immunization in this stud y suggest that further evaluation of this vaccination strategy is warranted .