An improved method for the analysis of sennosides in Cassia angustifolia by high-performance liquid chromatography

A reversed-phase column liquid chromatographic method for the analysis of s ennosides A and B present in leaf and pod extracts of Cassia angustifolia h as been developed using a Symmetry Cls column and a linear binary gradient profile. The method can be utilised for the quantitative determination of o ther sennosides as a baseline resolution for most of the constituents was a chieved, The method is economical in terms of the time taken and the amount of solvent used (25 mt) for each analysis. The validity of the method with respect to analysis was confirmed by comparing the UV spectra of each peak with those of reference compounds using a photodiode array detector. Copyr ight (C) 2001 John Wiley & Sons, Ltd.