A pilot scale two-stage batch diafiltration process for whey protein concen
trate (WPC) production is presented in this work. This process has two main
advantages: a significant water saving with respect to a single-stage diaf
iltration process and a membrane surface saving with respect to a continuou
s multistage process. Every unit operation of the process has been experime
nted in a pilot scale (ultrafiltration, diafiltration, drying), in order to
produce a WPC powder. Lactose content decreased from about 75% (of whey) t
o 4.5% (calculated as mass of lactose per total solute mass) and proteins i
ncreased from 15% to 83% (calculated as mass of proteins per total solute m
ass), with a water consumption of about 1.5 L/L of whey. Permeability tests
enabled the calculation of the main mass-transfer resistances through the
membrane: the intrinsic membrane resistance, R-M, was 13.8 +/-0.1, the one
due to fouling, R-F, was 3.68 +/-0.03, and the one due to polarization, R-G
, was estimated at 2.4 +/-0.4 (at 150 kPa TMP) 10(9) Pa s m(-1), at 25 degr
eesC. These values are in agreement with data reported in the literature, A
study performed on the membrane retention properties showed that not only
peptides but also two native proteins (beta -lactoglobulin and alpha -lacto
albumin) pass in the permeate during ultrafiltration. Consequently, a signi
ficant protein loss takes place during the process. Diafiltration tests per
formed in a two-stage countercurrent operation demonstrated the water savin
g with respect to a single-stage process (46%). Furthermore, experimental d
ata have been used in order to evidence the capability of a previously deve
loped mathematical model to predict the components' concentration during di
afiltration.