Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies

Fifty-seven monoclonal antibodies (mAb) selected after the first round anal yses in the Third International Swine CD workshop for their possible reacti vity with T-lymphocyte specific antigens were further analysed in a second round. As target cells for flow cytometric analyses served peripheral blood mononuclear cells, nylon-wool enriched T-lymphocytes, thymocytes, splenocy tes, and lymphocytes derived from Peyer's patches. These second round analy ses revealed 15 different data sets. Together with 22 pre-selected data set s from the first round analyses with the whole panel of monoclonal antibodi es, 37 data sets were used for the clustering of the respective mAb. Using the LTDB4 program, 19 preliminary clusters could be defined. Two clusters ( C3 and C7) with 4 mAb showed no labelling of resting T-lymphocytes. Seven c lusters (CI, C2, C4, C5, C6, C11, and C12) contain mAb (in total: 16 mAb) d irected against subsets of CD4(-)CD8(-) T-lymphocytes. These mAb seem to re cognise antigens on porcine T-lymphocytes with T-cell receptor (TcR) gamma/ delta chains. Three clusters (C8, C9, CIO, C13) seem to be artificial. They contain either mAb staining CD4(-)CD8(-) T-lymphocytes and low CD8+ cells (C8, C9), mAb with various reactivity (CIO) and mAb with known differences in their reactivity (C13). Cluster C14 contains 3 mAb against the CD4a-epit ope, C15 describes mAb directed against porcine CD8c-epitope whereas mAb ag ainst CD8a and CD8b-epitopes grouped in C19. The mAb found in C16 seem to r ecognise CD45R. Cluster C17 is composed of different standards directed aga inst CD2, CD3, CD5 and wCD6. Two additional mAb recognising the CD2a-epitop e could be enclosed. CIS contains two mAb directed against SWC2. (C) 2001 E lsevier Science B.V. All rights reserved.