Summary of workshop findings for porcine myelomonocytic markers

About 65 monoclonal antibodies (mAb) including 17 internal controls were an alyzed for their ability to recognize and bind to various cells of the myel omonocytic lineage. Flow cytometry (FCM) utilizing both single and double s taining, and immunoprecipitation (IP) assays were used in the analysis. Abo ut 38 of the mAb were reactive with myelomonocytic cells, resulting in nine clusters of interest. Although the exact identity of many of the molecules on the cells bound by the m-Ab remains undetermined, information obtained about the mAb analyzed in tn this workshop should be helpful in further ide ntifying various populations of myelomonocytic cells and their stages of di fferentiation. Out of 12 mAbs with potential CD11 specificity, seven were a ssigned to three different swine specific alpha chains of the CD11/CD18 int egrin heterodimer, the assignment of the remaining four was tentative. One antibody had a binding specificity consistent with SWC3 and one with SWC8. CD14 expression on pig cells was characterized with a panel of CD14-positiv e antibodies, two of these antibodies were assigned to swine CD14. Two anti bodies were assigned to CD163. Further work is required to determine the an tigens recognized by many of the other mAb. (C) 2001 Elsevier Science B.V. All rights reserved.