SEMIAUTOMATIC DNA PROFILING IN A HUNGARIAN ROMANY POPULATION USING THE STR LOCI HUMVWFA31, HUMTH01, HUMTPOX, AND HUMCSF1PO

A population study of Hungarian Romanies was carried out for the STR l oci HumVWFA31, HumTH01, HumTPOX, and HumCSF1PO. After multiplex PCR am plification semi-automatic DNA profiling was performed using an ALF DN A sequencer. At the loci investigated there was little and no evidence for departures from Hardy-Weinberg expectations and linkage equilibri um, respectively. The allele sizing accuracy of the ALF DNA sequencer was increased to a high level (99.97% on average) by applying external and internal markers. Allele frequency distributions of the STR loci, with one exception, were significantly different between the Romany a nd other Hungarian population databases. On the other hand, however, o nly small differences in frequencies of individual phenotypes were fou nd.