PREPARATION OF CHIRAL STATIONARY PHASES BY GRAFTING BETA-CD DERIVATIVES ON POLYVINYLIMIDAZOLE COATED SILICA

Several beta-cyclodextrin (beta-CD) chiral stationary phases (CSP) for HPLC were prepared, from silica beads coated with polyvinylimidazole (PVI). The functionalization carried out by reaction with 3-chloro-2-h ydroxypropyl beta-CD, gave rise to supports with varying content of be ta-CD cavities. The influence of the amount of beta-CD and of the aver age molecular weight of PVI on the resolution of D,L-methionine beta-n aphthylamide are studied for optimization. Results are compared to tho se obtained with a stationary phase prepared by coating silica with a beta-CD containing PVI preformed polymer. (C) 1997 Elsevier Science Lt d.