Use of capillary electrophoresis to quantitate carbamylated hemoglobin concentrations in dogs with renal failure

Objective-To evaluate quantification of the amount of carbamylated hemoglob in (CarbHb), using capillary electrophoresis (CE) and a new dynamic capilla ry coating system to separate hemoglobin derivatives, and to assess the use of CarbHb amounts to evaluate long-term urea exposure and differential dia gnoses of azotemia in dogs. Animals-8 dogs with renal failure, 2 dogs with diabetes mellitus, and 7 con trol dogs. Procedure Optimal analytic conditions for separation of CarbHb and other he moglobin derivatives in blood samples obtained from dogs were determined, u sing a commercial analysis system developed for the detection of glycohemog lobin Hb A1c (GlycHb) in human blood samples. Relative content of hemoglobi n derivatives in blood from 10 dogs with renal failure or endocrine disease s were compared with values for 7 dogs without renal or endocrine diseases. Results Satisfactory resolution of hemoglobin derivatives was obtained, whi ch permitted identification and quantitation of the amount of CarbHb as a p ercentage of the total amount of hemoglobin. Normal or increased amounts of GlycHb did not interfere with CarbHb analysis. Dogs with chronic renal fai lure had considerably higher peak amounts of CarbHb than dogs with acute re nal failure, a dog with chronic renal failure that was treated by use of he modialysis, or dogs without renal disease. Conclusions and Clinical Relevance Amounts of CarbHb in blood samples obtai ned from dogs can be readily quantified by use of capillary electrophoresis . Assessment of the amount of CarbHb can be used to facilitate evaluation o f the cause of azotemia in dogs.