Synthesis and antiproliferative activity in vitro of new derivatives of 3-aminopyrazolo[3,4-b]pyridine - Part 1. Reaction of 3-aminopyrazolo[3,4-b]pyridine with 1,3-, 1,4-diketones and alpha,beta-unsaturated ketones

The synthesis of several new pyrazolo[3,4-b]pyridine, pyrido[2',3':3,4]-pyr azolo[ 1,5-a]pyrimidine and imidazo[1',2': 1,5]pyrazolo[3,4-b]pyridine deri vatives is described. The obtained compounds were tested for their antiprol iferative activity in vitro. One of thetas, 4-phenyl-2-(3,4,5-trimethoxy-be ta -styrylo)pyrido[2',3':3,4]pyrazolo[ 1,5-a]pyrimidine {9), revealed cytot oxic properties against the cells of all three human cancer cell lines appl ied. Another one, 2,4-dimethyl-pyrido[2',3':3,4]pyrazolo[1,5-a]pyrimidine { 2), revealed weak cytotoxic activity only against the cells of human bladde r cancer cell line HCV29T. All other compounds tested did not reveal any cy totoxic activity.