Feeding strategy of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Ralstonia eutropha

The effects of propionic acid feeding strategy on production of poly(3-hydr oxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] were studied with Ralston ia eutropha. Flask culture revealed that the time and concentration of prop ionic acid addition had significant effects on cell growth, P(3HB-co-3HV) s ynthesis, and HV fraction in the copolymer. In fed-batch culture, a low rat io of propionic acid to glucose (P/G) led to high dry cell weight (DCW), P( 3HB-co-3HV) content and productivity, but low HV unit fraction. A high P/G ratio led to, on the other hand, high HV unit fraction but the low P(3HB-co -3HV) content and productivity. The specific P(3HB-co-3HV) synthetic rate a nd the specific HV synthetic rate declined in fed-batch cultures, which det eriorated with high P/G feeding due to the inhibitory effect of propionic a cid accumulated in the culture broth. According to the non-steady state syn thesis of P(3HB-co-3HV) by R. eutropha, an optimal feeding strategy to cont rol the propionic acid accumulation was developed and demonstrated. The pro pionic acid feeding rate was reduced with time, and DCW, P(3HB-co-3HV) conc entration, P(3HB-co-3HV) content and RV fraction in P(3HB-co-3HV) reached 5 2.1 gl(-1), 40.8 gl(-1), 78.3% and 16.2 mol%, respectively. P(3HB-co-3HV) p roductivity was 0.74 gl(-1) h(-1). (C) 2001 Elsevier Science B.V. All right s reserved.