Purification of saponin compounds in Bupleurum falcatum by solvent partitioning and preparative LC

Authors
Kim, N Park, IS
Citation
N. Kim et Is. Park, Purification of saponin compounds in Bupleurum falcatum by solvent partitioning and preparative LC, BIOS BIOT B, 65(7), 2001, pp. 1648-1651
Citations number
13
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
65
Issue
7
Year of publication
2001
Pages
1648 - 1651
Database
ISI
SICI code
0916-8451(200107)65:7<1648:POSCIB>2.0.ZU;2-G
Abstract
Saponin compounds (saikosaponin c, a, and d) in Bupleurum falcatum were par tially purified by solvent partitioning of the herbal extract using diethyl ether, distilled water, n-butanol, and acetone. After separation of the sa ponins by preparative LC, the purity of each saikosaponin was more than 94% . The identities of purified individual saikosaponins were confirmed by TLC , analytical LC, and fast-atom bombardment mass spectrometry.