Cross-linking study of cytochrome P450 1A2 in proteoliposomes

Authors
Myasoedova, KN Magretova, NN
Citation
Kn. Myasoedova et Nn. Magretova, Cross-linking study of cytochrome P450 1A2 in proteoliposomes, BIOSCI REP, 21(1), 2001, pp. 63-72
Citations number
32
Categorie Soggetti
Cell & Developmental Biology
Journal title
BIOSCIENCE REPORTS
ISSN journal
01448463 → ACNP
Volume
21
Issue
1
Year of publication
2001
Pages
63 - 72
Database
ISI
SICI code
0144-8463(200102)21:1<63:CSOCP1>2.0.ZU;2-B
Abstract
Proteoliposomes, containing cytochrome P450 1A2, were obtained by the chola te-dialysis technique. The effect of bifunctional cross-linking reagents on the purified hexameric cytochrome P450 1A2 in an aqueous medium and on the proteoliposomal P450 1A2 have been compared. Electrophoretic analysis of t he modified proteins demonstrated the same oligomeric (hexameric) organizat ion of the hemoprotein in each case.