New and effective entrapment of polyelectrolyte-enzyme-complexes in LentiKats

Authors
Czichocki, G Dautzenberg, H Capan, E Vorlop, KD
Citation
G. Czichocki et al., New and effective entrapment of polyelectrolyte-enzyme-complexes in LentiKats, BIOTECH LET, 23(16), 2001, pp. 1303-1307
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
16
Year of publication
2001
Pages
1303 - 1307
Database
ISI
SICI code
0141-5492(200108)23:16<1303:NAEEOP>2.0.ZU;2-R
Abstract
Amyloglucosidase EC 3.2.1.3 was used as model for the immobilisation of enz ymes in poly(vinylalcohol) hydrogel (LentiKats) lenses. The entrapment of t he enzyme in PVA-hydrogel based on a two-step procedure, firstly its coupli ng to polyelectrolytes increased the structure level of the enzyme, and sub sequently, the resulting complex was entrapped in LentiKats. The immobilise d enzyme retained 45% of its original activity and lost no activity over fi ve repeated batch runs.