Cloning and prokaryotic expression of HGLP cDNA

A novel cDNA, HGLP, which encodes a G-protein coupled receptor (GPCR) like protein, has been isolated and cloned. The coding region of the human HGLP predicts a 7-transmembrane region protein with motifs of rhodopsin-like GPC R superfamily. Northern blot analysis reveals a 3-kb transcript in various human tissues examined. The N- and C-terminal coding regions of HGLP, which are deduced as non-transmembrane regions, have been amplified by PCR and c loned into pET30a(+) vector. Then the recombinant proteins are highly expre ssed in E. coli.