Crystallization and preliminary X-ray crystallographic analysis of yeast prion protein Ure2p with shortened N-terminal

Authors
Wang, LW Zhu, L Zhou, JM Rao, ZH
Citation
Lw. Wang et al., Crystallization and preliminary X-ray crystallographic analysis of yeast prion protein Ure2p with shortened N-terminal, CHIN SCI B, 46(12), 2001, pp. 1012-1014
Citations number
19
Categorie Soggetti
Multidisciplinary
Journal title
CHINESE SCIENCE BULLETIN
ISSN journal
10016538 → ACNP
Volume
46
Issue
12
Year of publication
2001
Pages
1012 - 1014
Database
ISI
SICI code
1001-6538(200106)46:12<1012:CAPXCA>2.0.ZU;2-E
Abstract
An orthorhombic crystal form of a recombinant yeast prion protein with shor tened N-terminaI, 90Ure2p, has been obtained. Crystals were grown by the va pordiffusion technique against a mother liquor containing imidazole. Crysta ls belong to the primitive orthorhombic lattice with the cell parameters a = 54.5 Angstrom, b = 74.7 Angstrom, c = 131.0 Angstrom. The crystals diffra ct to beyond 3.0 Angstrom resolution at a synchrotron beamline.