Temperature-dependent separation of bryostatin 18 and 10 by high performance liquid chromatography

Authors
Lu, F Yang, GJ Liu, LL Shen, XF Lin, HW Yi, YH
Citation
F. Lu et al., Temperature-dependent separation of bryostatin 18 and 10 by high performance liquid chromatography, CHROMATOGR, 54(1-2), 2001, pp. 57-59
Citations number
11
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
54
Issue
1-2
Year of publication
2001
Pages
57 - 59
Database
ISI
SICI code
0009-5893(200107)54:1-2<57:TSOB1A>2.0.ZU;2-4
Abstract
The temperature-dependent separation of bryostatins by HPLC was examined on an octadecyl bonded stationary phase, using column temperatures between 0 and 40 degreesC and mobile phase temperatures from 0 to 25 degreesC. The re tention time and resolution of bryostatins changed drastically and separati on improved with decreasing temperature. A column temperature of less than 5 degreesC and a mobile phase temperature of less than 15 degreesC is recom mended for a good resolution of bryostatins for routine work.