ADENOVIRUS ISOLATION FROM SPLEEN LYMPHOCYTES OF APPARENTLY HEALTHY PIGS

A polyethylene glycol treatment was given to fuse KSEK6 cells, an esta blished cell line derived from porcine embryo kidney, with the lymphoc ytes, separated from spleens of 35 apparently healthy slaughtered pigs . Eight cytopathic virus strains were isolated from the lymphocytes of these pigs. Two virus strains were isolated by inoculating the spleen tissue homogenates to KSEK6 monolayer cultures. All of viruses were i dentified as porcine adenoviruses according to their physicochemical, serological and immunological properties. One of these virus strains w as serologically proved to be independent from six serotypes of porcin e adenoviruses ever known. The electrophoretic property of viral DNA o f this strain was indicated to be different from those of other refere nce porcine adenoviruses. This means the presence of a 7th serotype in porcine adenoviruses.