Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression

Authors
Moutou, KA Socorro, S Power, DM Mamuris, Z Canario, AVM
Citation
Ka. Moutou et al., Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression, COMP BIOC B, 130(1), 2001, pp. 13-21
Citations number
20
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN journal
10964959 → ACNP
Volume
130
Issue
1
Year of publication
2001
Pages
13 - 21
Database
ISI
SICI code
1096-4959(200108)130:1<13:MCASOG>2.0.ZU;2-G
Abstract
In the present study, the Sparus aurata alpha -skeletal actin was cloned fr om a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid pro tein. The deduced amino acid sequence of sea bream a-actin is identical to Fugu alpha -actin-1. The expression of a-actin was initiated at the onset o f segmentation. In adult fish, alpha -actin is expressed predominantly in w hite and red muscle. (C) 2001 Elsevier Science Inc. All rights reserved.