Uveitopathogenic sites in recoverin

Purpose. This study was carried out in order to determine the most potent a nd novel uveitopathogenic sites of recoverin using synthetic peptides. Methods. Several synthetic peptides containing the recoverin sequence plus adjuvants were injected into Lewis rats, and the uveitopathogenic sequence was defined, clinically, histologically, and immunologically. Results. Peptides containing of amino acids 57-85 and 136-167 induced sever e EAU, and the lowest doses to induce EAU were 20 mug and 10 mug, respectiv ely. Lymphocyte proliferative reactions were also positive for peptides 57- 85 and 136-167. The core sequences within the uveitopathogenic site were 65 -79 and 153-164. Peptides of amino acids 65-79 within 57-85 and 149-167 wit hin 136-167 were the smallest in the recoverin sequence, respectively, that could induce severe EAU. Conclusion. We found recoverin has some novel potent uveitopathogenic sites , 149-167. These findings of the uveitopathogenic sites in recoverin may le ad to improved understanding of the pathogenesis of uveitis and the means t o design specific treatment.