A novel ormosil based electro catalytic biosensor for glucose/ethanol based on dehydrogenase modified electrode

A novel ormosil material for designing electrocatalytic biosensors for gluc ose and ethanol based on dehydrogenase catalyzed reactions is reported. The electrode material is prepared using palladium-linked glycidoxypropyltrime thoxysilane, ferrocene monocarboxylic acid, trimethoxysilane and HCl. The o rmosil prepared from these ingredients shows reversible electrochemistry of ormosil encapsulated ferrocene. The electrocatalytic oxidation of NADPH/NA DH and subsequently novel dehydrogenase based biosensors for glucose and et hanol are developed. The results based on cyclic voltammetry and amperometr y are reported. The enzyme sensors are developed based on the modification of electrode material by dehydrogenase enzymes. The modification of electro de material is made by two approaches of enzyme immobilization: immobilizat ion of dehydrogenase within polyvinyl alcohol and sandwiching the dehydroge nase within two layers of ormosils among which the first layer being the el ectrode material itself and the second layer of ormosil prepared without pa lladium linkage precursor and ferrocene. The biosensor made by the second a pproach shows high stability and much better reproducibility of enzyme elec trode performance. The results on glucose and alcohol sensing based on elec trochemical measurements are reported.