Crucial amino acid residues of mouse CD1d for glycolipid ligand presentation to V(alpha)14NKT cells

Citation
N. Kamada et al., Crucial amino acid residues of mouse CD1d for glycolipid ligand presentation to V(alpha)14NKT cells, INT IMMUNOL, 13(7), 2001, pp. 853-861
Citations number
42
Categorie Soggetti
Immunology
Journal title
INTERNATIONAL IMMUNOLOGY
ISSN journal
09538178 → ACNP
Volume
13
Issue
7
Year of publication
2001
Pages
853 - 861
Database
ISI
SICI code
0953-8178(200107)13:7<853:CAAROM>2.0.ZU;2-F
Abstract
A novel lymphocyte, NKT cells bearing an invariant V(alpha)14 antigen recep tor, specifically recognizes alpha -galactosylceramide (alpha -GalCer) excl usively presented by mouse CD1d (mCD1d). However, the precise molecular int eraction remains unclear. For the basis of functional analyses, a docking m odel of alpha -GalCer with the crystal structure of mCD1d was constructed. Possible residues involved in the alpha -GalCer-mCD1d interaction were foun d to be Arg79, Glu83 and Asp80 for carbohydrate recognition, and Asp153 for interaction with the amide group on the fatty acyl chain. The alpha -GaLCe r-presenting ability of various transfectants expressing mutant mCD1d was c ompletely abrogated it a single amino acid mutation was induced at position s 79, 80, 83 or 153, suggesting that the polar amino acids above the F ' po cket are crucial for alpha -GalCer presentation to activate V alpha 14 NKT cells. The possibility that Glu83 is a contact site for the NKT cell recept or is also discussed.