Identification of a new imprinted gene, Rian, on mouse chromosome 12 by fluorescent differential display screening

Systematic screening of differentially expressed genes among androgenetic, parthenogenetic, and normal embryos by means of fluorescent differential di splay revealed five imprinted genes. One of them, named Rian, was expressed exclusively from the maternal allele and was closely linked to an imprinte d gene, Meg3(Gtl2), mapped to the distal end of chromosome 12. The Rian tra nscript did not have any apparent open reading frame, and its transcript wa s exclusively localized to the nucleus.