Identification and synthesis of a male-produced sex pheromone from the stink bug Chlorochroa sayi

The reproductive behavior of the stink bug Chlorochroa sayi was studied in the laboratory. There was a sexual maturation period of about 10 days befor e bugs began mating. Sexually mature adult bugs engaged in courtship consis ting of antennation and head-butting of the female by the male, before the female adopted a receptive posture and copulation occurred. Both sexes mate d multiple times during their life-spans, with the mean duration of copulat ions of virgin bugs (42.3 +/- 19.6 min) and experienced bugs (37.3 +/- 28.4 min) being similar. Most matings were initiated in the late afternoon or e vening, when pheromone production by males was greatest. Males transferred sperm and nutrients constituting about 17% of their body weight to females during mating. Three male-specific components, methyl geranate, methyl citr onellate, and methyl (E)-6-2,3-dihydrofarnesoate in a ratio of 100 : 0.45 : 1.6, were first detected in volatiles collected from male bugs on green be ans about 9-12 days after the final molt to the adult stage. In vertical Y- tube bioassays, females were attracted to odors from mature male bugs, and to a blend of the three male-produced components. Low numbers of females al so were attracted in field trials with the three-component blend. The relat ively weak attraction may be a result of other, as yet unknown cues being r equired in addition to the pheromone, such as visual or substrate-borne vib rational cues.