Separation of glyceride positional isomers by silver ion chromatography

Separation of triglyceride and diglyceride positional isomers by silver ion high-performance liquid chromatography coupled with an evaporative light-s cattering detector is described. The triglyceride isomers had a fatty acid composition of CLC and CCL, where C and L were caprylic acid and linoleic a cid, respectively. Diglyceride isomers, 1,2(2,3)-diglyceride and 1,3-diglyc eride, which contained caprylic acid were separated too. A solvent system b ased on n-hexane, 2-propanol, ethyl acetate, and acetonitrile with a flow-r ate of 0.8 ml/min was developed. Calibration curves of CLC and CCL were ach ieved with triolein as internal standard. Using this method, the incorporat ion of linoleic acid onto specific a position of glycerol backbone can be m onitored. (C) 2001 Elsevier Science B.V. All rights reserved.