Preparation and characterization of monolithic polymer columns for capillary electrochromatography

A series of micro-monolithic columns with different porosities were prepare d for capillary electrochromatography (CEC) by in-situ copolymerization of butyl methacrylate, ethylene glycol dimethacrylate, and 2-acrylamido-2-meth yl-1-propanesulfonic acid in the presence of a porogen in fused-silica capi llaries of 100 mum I.D. Different column porosities were obtained by changi ng the ratios of monomers to porogenic solvents. Columns were investigated and evaluated under both pressure-driven (high-performance liquid chromatog raphy, HPLC) and electro-driven (capillary electrochromatography, CEC) cond itions. Each column exhibited different efficiency and dependency on flow v elocity under electro-driven conditions. Abnormally broad peaks for some re latively bulky molecules were observed. Possible explanations are discussed . The differences in column efficiency and retention behavior between the t wo eluent-driven modes were studied in detail. In addition, other column pr oper-ties, such as morphology, porosity, stability and reproducibility, wer e extensively tested. (C) 2001 Elsevier Science B.V. rights reserved.