Quantitative determination of amygdalin epimers by cyclodextrin-modified micellar electrokinetic chromatography

A new capillary electrophoresis method was developed for the quantitative d etermination of the amygdalin epimers, amygdalin and neoamygdalin, which ar e biologically significant constituents in the crude drugs, namely Persicae Semen and Armeniacae Semen. The effects of surfactants, additives and othe r analytical parameters were studied. As a result, the resolution of two ep imers was performed by cyclodextrin-modified micellar electrokinetic chroma tography with a buffer containing alpha -cyclodextrin and sodium deoxychola te. By the application of this method, a simple. fast and simultaneous quan titative determinations of amygdalin epimers in the crude drugs (Persicae S emen and Armeniacae Semen) and the Chinese herbal prescriptions (Keishi-buk uryo-gan and Mao-to) were achieved. (C) 2001 Elsevier Science BY. All right s reserved.