L. Chen et al., Cariogenic actinomyces identified with a beta-glucosidase-dependent green color reaction to Gardenia jasminoides extract, J CLIN MICR, 39(8), 2001, pp. 3009-3012
The oral bacteria Actinomyces naeslundii and Actinomyces viscosus are known
to contribute to the initiation and progression of human dental caries, es
pecially root caries. We report that both A. naeslundii and A. viscosus rea
ct with a component in the Gardenia jasminoides extract to produce a distin
ct green product. This green color reaction was found to be dependent on th
e bacterial beta -glucosidase. The reaction is specific for cariogenic acti
nomyces, and it can detect as few as 10(4) cells of A. naeslundii and A. vi
scosus per ml.