Phase II clinical investigation of gemcitabine in advanced soft tissue sarcomas and window evaluation of dose rate on gemcitabine triphosphate accumulation

Purpose: To evaluate the efficacy, toxicity, and optimal dose rate of gemci tabine in adult patients with advanced soft tissue sarcomas (STS) by compar ing levels of gemcitabine triphosphate (GTP) in peripheral-blood mononuclea r cells (PBMCs) of patients receiving two different dose rates. Patients and Methods: Fifty-six assessable patients with STS (17 gastrointe stinal [GI] leiomyosarcomas and 39 other histologies) were treated on a two -arm phase 11 study. Gemcitabine was given at 1 g/m(2) as a 30-minute infus ion weekly for up to 7 weeks followed by I week of rest and reassessment of tumor. Subsequent cycles were given at 1 g/m(2) weekly for 3 weeks followe d by 1 week of rest. Nine patients underwent cellular pharmacologic studies at two different dose rates (1 g/m(2) over a standard 30-minute infusion o n week I and over pharmacologically based infusion of 150 minutes on week 2 ) to evaluate GTP levels in PBMCs. Results: Seven partial responses were noted among 39 patients, for an overa ll response rate of 18% (95% confidence interval, 7% to 29%). Median durati on of response was 3.5 months (range, 2 to 13 months). Four of 10 patients with non-GI leiomyosarcomas achieved a partial response. No objective respo nses were noted in 17 patients with GI leiomyosarcomas. One patient had a m ixed response. Median time to progression for all patients (both arms) was 3 months; median survival was 13.9 months. Treatment was generally well tol erated. Comparison of cellular pharmacology demonstrated a significant 1.4- fold increase in the concentration of GTP with the 150-minute infusion. Conclusion: Given the limited therapeutic armamentarium for STS, the activi ty of gemcitabine is encouraging. Its potential for combination therapy in the salvage setting should be studied with pharmacologically guided fixed d ose-rate infusion.