Tl. Chan et al., Bipolar cell diversity in the primate retina: Morphologic and immunocytochemical analysis of a New World monkey, the marmoset Callithrix jacchus, J COMP NEUR, 437(2), 2001, pp. 219-239
The aim of this study was to identify the bipolar cell types in the retina
of a New World monkey, the common marmoset, and compare them with those fou
nd in the Old World macaque monkey. Retinal whole-mounts, sections, or both
, were stained by using DiI labeling and immunohistochemical methods. Semit
hin sections were analyzed by using quantitative methods. We show that the
same morphologic types of bipolar cell as described for the Old World macaq
ue monkey by Boycott and Wassle (Boycott and Wassle [1991] Eur. J. Neurosci
. 3:1069-1088) are present in marmoset retina: two types of midget bipolar
cells, six type of diffuse bipolar cells, a blue cone bipolar cell, and one
type of rod bipolar cell. The pattern of staining with different immunohis
tochemical markers ("fingerprint") of each bipolar cell type in marmoset wa
s also the same as described for macaque, with one exception: the flat midg
et bipolar cell (FMB) class is labeled by antibodies to recoverin in macaqu
e but is labeled by antibodies to CD15 in marmoset. The labeled FMB cells i
n marmoset make contact with multiple cone photoreceptors throughout most o
f the extrafoveal retina. The spatial density of bipolar cells in marmoset
is shown to be sufficient to support one-to-one connectivity of midget bipo
lar and ganglion cells in the fovea and to allow for parallel pathways to g
anglion cells throughout the retina. Quantitative differences in the morpho
logy and receptor connectivity between marmoset and macaque can be related
to differences in cone and rod photoreceptor density between the species. W
e conclude that bipolar cell diversity is a preserved feature of the primat
e retina. (C) 2001 Wiley-Liss, Inc.