Effects of phosphorothionate on the reproductive system of male rats

Acute and Sub-acute toxic effects of a new pesticide phosphorothionate code d as RPR-V on testis of albino rat were studied. For the acute study, rats received a single dose of 30 mg/kg of RPR-V and sacrificed after 24 hours. For the Subacute study, 1.42 mg/kg/day was administered orally to rats for 10 days and 21 days. Acute exposure of rats to RPR-V brought no change eith er in the Gonadosomatic Index (GSI) or in the structure of testis or in the serum levels of Testosterone. Similarly, no significant change was observe d in the Glutathione S-transferase (GST) activity. But, in testis there was significant increased in the reduced Glutathione (GSH) and Acid Phosphatas e (AcP), whereas Alkaline Phosphatase (AkP) levels decreased significantly at 24hr post treatment. On 7(th) day (withdrawal period) after the cessatio n of the treatment the GSH, AcP, and AkP levels reached to near control. Th e sub-acute study revealed a significant post treatment. Due to RPR-V treat ment the testis AcP levels increased significantly at 21(st) day of medicat ion but AkP levels decreased both at 10(th) and 21(st) day of post treatmen t. Histopathological studies showed that after 10(th) day testis showed con siderable loss of spermatozoids and at 21(st) day complete derangement of c ellular organization was observed. Testosterone levels decreased significan tly after 10th day and remained significantly low at 21(st) day. However, w ithdrawal studies showed a recovery in testis of rat treated with RPR-V. GS T, GSH, GSI, AcP and AkP values were recovered, testosterone levels were al so recovered but recovery in testis structure remained at a low profile.