Role of poly(ADP-ribose) synthetase activation in the development of experimental allergic encephalomyelitis

Peroxynitrite formation has been demonstrated during experimental allergic encephalomyelitis (EAE). Furthermore, peroxynitrite has been identified as an activator of poly(ADP-ribose) synthetase (PARS). an enzyme implicated in neurotoxicity. In the current study, we examined the role of PARS activati on in the development of EAE. Administration of the PARS inhibitor 5-iodo-6 -amino-1,2-benzopyrone (INH2-BP) delayed the onset of EAE and reduced the i ncidence and severity of disease signs. Moreover, drug treatment lowered iN OS activity and decreased cell infiltration in cervical spinal tissues from EAE-sensitized animals. To conclude, the results of the present investigat ion suggest that PARS activity may contribute to the pathogenesis of EAE. ( C) 2001 Published by Elsevier Science B.V.