Theiler's virus-infected L-selectin-deficient mice have decreased infiltration of CD8(+) T lymphocytes in central nervous system but clear the virus

Mice with targeted deletion of L-selectin gene (L-sel(-/-)) were used to in vestigate the role of adhesion molecule in immunologic responses following virus infection in the central nervous system (CNS). L-Sel(-/-) mice from a resistant H-2(b) genetic background and parental wild-type H-2(b) (C57BL/6 ) mice were infected with Theiler's murine encephalomyelitis virus (TMEV) i ntracerebrally and the kinetics of virus replication and infiltration of im mune cells in the CNS determined. The levels of infectious TMEV, as measure d by plaque assay at 3, 7, 14, and 28 days after infection were between 4 a nd 6 log(10) to PFU of virus per gram of CNS tissues at days 3 and 7 post-i nfection, and then decreased to undetectable levels by day 14 after infecti on in both strains of mice. The L-sel(-/-) mice had decreased numbers of CD 8(+) T lymphocytes (17.72% +/- 2.4) infiltrating into the CNS at 7 days pos t-infection when compared to wild-type mice (31.02% +/- 7.5). In addition, the L-sel(-/-) mice had significantly lower levels of TMEV-specific serum I gG resulting in lower virus neutralizing activity of the serum when compare d to wild-type mice. However, the L-sel(-/-) mice had 2.5-fold increase in B lymphocytes in the CNS (8.29% +/- 1.1) when compared to wild-type mice (3 .2% +/- 0.4). Taken together, these data indicate that L-selectin plays a r ole in recruitment of B and CD8(+) T lymphocytes into the CNS following vir us infection, which, however, did not affect the ability of the mice to cle ar TMEV infection. (C) 2001 Elsevier Science B.V. All rights reserved.