Variable galactocerebroside expression by human Schwann cells in dissociated and peripheral nerve explant cultures

Citation
Vj. Turnbull et al., Variable galactocerebroside expression by human Schwann cells in dissociated and peripheral nerve explant cultures, J NEUROSC R, 65(4), 2001, pp. 318-321
Citations number
17
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROSCIENCE RESEARCH
ISSN journal
03604012 → ACNP
Volume
65
Issue
4
Year of publication
2001
Pages
318 - 321
Database
ISI
SICI code
0360-4012(20010815)65:4<318:VGEBHS>2.0.ZU;2-G
Abstract
Its has been well established that rat Schwann cells down regulate their ce ll-surface expression of galactocerebroside (GalC) in vitro under normal ce ll culture conditions. To determine whether human Schwann cells exhibit a s imilar down-regulation of GalC in vitro we examined GalC expression in diss ociated human Schwann cell cultures derived from normal adult peripheral ne rve. Twenty-four hours post-dissociation up to 63% of human Schwann cells w ere found to express detectable levels of GalC on their surface whereas les s than 8% of the Schwann cells expressed detectable levels of GalC at 14 da ys post-dissociation. In contrast, after nearly 3 months of peripheral nerv e explant culture, greater than 30% of human Schwann cells still retained t heir GalC expression. A similar pattern was also observed when analyzing Sc hwann cell purity with dissociated cultures exhibiting a rapid decrease in Schwann cell purity under normal culturing conditions although Schwann cell purity was found to be largely unaffected during the period of peripheral nerve explant culture. In summary, we found there was less variation in bot h GalC expression and Schwann cell purity with time in peripheral nerve exp lant cultures than dissociated cultures. (C) 2001 whey-hiss, Inc.