Malt containing high levels (up to 52 mug/kg) of ochratoxin A (OA) was prep
ared on a pilot scale by inoculating malting barley with live Penicillium v
errucosum. This malt was then used for pilot brewing trials to determine th
e extent to which OA can survive the brewing process. Substantial losses, u
p to 40% of the OA in the grist, were observed during mashing, most probabl
y by proteolytic degradation. Another 16% was eliminated with the spent gra
ins. Some losses of OA occurred during fermentation, but these were less th
an those in mashing. Overall, between 13 and 32% of the OA present in the o
riginal gist survived into the beer.