Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement

Authors
Huai, Q Colandene, JD Topal, MD Ke, HM
Citation
Q. Huai et al., Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement, NAT ST BIOL, 8(8), 2001, pp. 665-669
Citations number
27
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NATURE STRUCTURAL BIOLOGY
ISSN journal
10728368 → ACNP
Volume
8
Issue
8
Year of publication
2001
Pages
665 - 669
Database
ISI
SICI code
1072-8368(200108)8:8<665:SONCRD>2.0.ZU;2-5
Abstract
Nael, a novel DNA endonuclease, shows topoisomerase and recombinase activit ies when a Lys residue is substituted for Leu 43. The Nael-DNA structure de monstrates that each of the two domains of Nael recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 Angstrom to grip DNA, widening that of t he Endo domain 8 Angstrom to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The Nael-DNA structure presents the first example of novel recognition of two copies of one DNA se quence by two different amino acid sequences and two different structural m otifs in one polypeptide.