Sc. Burton et Drk. Harding, BIFUNCTIONAL ETHERIFICATION OF A BEAD CELLULOSE FOR LIGAND ATTACHMENTWITH ALLYL BROMIDE AND ALLYL GLYCIDYL ETHER, Journal of chromatography, 775(1-2), 1997, pp. 29-38
Citations number
39
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Efficient carboxymethylation of a bead cellulose was achieved with dim
ethyl sulphoxide-water mixtures but low activation levels were found w
ith epoxidation reagents. The highest activation levels were obtained
with allyl bromide and allyl glycidyl ether. This was attributed to th
e relative inertness of the allyl group towards water and cellulose, l
imiting hydrolysis and crosslinking. Advantages over epoxidation chemi
stry for bead cellulose were efficient reagent use, reproducible activ
ation up to high levels and specificity of matrix modification. Predic
table activation was obtained and facile titration methods were develo
ped. Efficient activations of agarose and methacrylate matrices were a
lso obtained with the allyl reagents.