Sc. Burton et Drk. Harding, HIGH-DENSITY LIGAND ATTACHMENT TO BROMINATED ALLYL MATRICES AND APPLICATION TO MIXED-MODE CHROMATOGRAPHY OF CHYMOSIN, Journal of chromatography, 775(1-2), 1997, pp. 39-50
Citations number
40
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Allylated cellulose, agarose and methacrylate matrices, activated with
allyl bromide or allyl glycidyl ether, were modified by aqueous bromi
nation, preferably with N-bromosuccinimide. Amine, thiol and sulphite
ligands were attached efficiently and in high densities (1-1.5 mmol/g
dry) to the brominated matrices, using concentrated reaction mixtures.
Organic solvents were not required. Bromohydroxypropyl matrices and a
mine or carboxylate derivatives could thus be used to produce matrices
for all major forms of adsorption chromatography. This chemistry was
used to prepare high-density mercaptoalkyl acid matrices, which were s
uccessfully applied to mixed mode purification of crude chymosin and c
ompared with matrices used previously. (C) 1997 Elsevier Science B.V.