Astrocytes induce manganese superoxide dismutase in brain capillary endothelial cells

Astrocytes induce blood-brain barrier (BBB) proper-ties in brain endothelia l cells (EC). O-2, generated in blood and EC, opens the BBB. Hence, high ac tivity of superoxide dismutase (SOD) is a prerequisite for normal BBB funct ion. Therefore, the influence of rat astrocytes on the expression of mangan ese (Mn)SOD in rat EC was investigated in two coculture models of the BBB, allowing either exchange of soluble factors or additionally cellular contac ts, Activity, protein content and mRNA expression of endothelial MnSOD were significantly increased in both coculture models in comparison to monocult ure by soluble astrocytic factors, such as cytokines. High activity of endo thelial MnSOD may be considered as a further essential property of the BBB, which is induced and maintained by astrocytes. NeuroReport 12:2513-2517 (C ) 2001 Lippincott Williams & Wilkins.