Homologs of the genes for receptor-like kinase proteins conferring plant resistance to pathogens. Comparative analysis of the homologs of the wheat gene Cre3 in the triticeae

Direct genomic DNA amplification with the primers recognizing the NBS-kinas e sequence of the wheat gene Cre3 (Genbank accession AF052641) was used to obtain partial homologs of this gene in perennial and annual rye, wheat, an d tail wheatgrass. The nucleotide sequences of the cloned fragments and the ir deduced amino acid sequences were compared to the already-known Cre3 hom ologs in other wheat, aegilops, and barley genotypes. Within the tribe Trit iceae, the extent of homology ranged from 86 to 94% for nucleotide sequence s and from 74 to 96% for the deduced amino acid sequences, with the most va riable region between Kin3 and PR3 conserved motifs.