DETERMINATION OF HOMOSERINE KINASE-ACTIVITY BY CHROMATOGRAPHIC-SEPARATION AND MEASUREMENT OF REACTION-PRODUCTS

Authors
GENING LV ANDREEVA NB GAZARYAN TG KHROMOV IS GAZARYAN KG
Citation
Lv. Gening et al., DETERMINATION OF HOMOSERINE KINASE-ACTIVITY BY CHROMATOGRAPHIC-SEPARATION AND MEASUREMENT OF REACTION-PRODUCTS, Biochemistry, 59(8), 1994, pp. 923-928
Citations number
6
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
59
Issue
8
Year of publication
1994
Pages
923 - 928
Database
ISI
SICI code
0006-2979(1994)59:8<923:DOHKBC>2.0.ZU;2-R
Abstract
The activity and kinetic characteristics of both purified and unpurifi ed homoserine kinase can be determined by thin-layer and paper chromat ography of labeled reaction products followed by measurement of radioa ctivity of the spots. The procedure is simpler, more sensitive, and mo re specific than the methods used to date.