Total protein and isozyme characterization in the flax zygotic embryo during development

Flax zygotic embryogenesis was studied for isozyme patterns of acid phospha tase and esterase and for content of total proteins. For acid phosphatase, six multiple molecular forms or isozymes, were identified during flax zygot ic embryo development. For esterase, six isozymes were expressed during zyg otic flax embryogenesis. Some of the isozymes were expressed during entire embryogenesis, and some were only transiently expressed or appeared near ma turation. The amount of total proteins was very low at early embryogenesis and proteins in a range from 26.6 kDa to 97.4 kDa were expressed. During fu rther flax embryo development the protein spectra became more diverse and t he quantity of total proteins increased. Proteins ranging from 3.4 kDa to 9 7.4 kDa were present, and proteins of 20 kDa to 36.5 kDa were expressed in the highest amount. The results are discussed with reference to selected in formation in the literature.