T. Eaves-pyles et Jw. Alexander, Comparison of translocation of different types of microorganisms from the intestinal tract of burned mice, SHOCK, 16(2), 2001, pp. 148-152
Citations number
35
Categorie Soggetti
Aneshtesia & Intensive Care","Cardiovascular & Hematology Research
The aim of this study was to compare the ability of various microorganisms
to translocate from the intestine to the mesenteric lymph nodes (MLNs), liv
er, and spleen in a burned mouse model. Balb/c mice were gavaged with 1 x 1
09 or 1 x 10(10) of one of 11 different microorganisms. All animals were th
en given a 20% burn. Survival after 10 days showed no significant differenc
e between any of the groups at the 1010 dose. At the 109 dose, significantl
y higher survival rates were found in three of the 11 strains. Microbial tr
anslocation (gavage of 1010 In-111-labeled organisms) and host's ability to
kill translocated bacteria (viable bacteria in tissues) were measured foll
owed by burn injury and sacrifice four hours later. Translocation and killi
ng of Staphylococcus epidermidis and Escherichia coli was high in the MLNs
compared with all other groups but translocation was lower to the liver. Kl
ebsiella, Pseudomonas, and Serratia translocated more evenly to all the tis
sues. However, these groups showed very high clearance of bacteria in the l
iver and spleen except for Klebsiella and one strain of Pseudomonas in the
spleen. Candida showed poor translocation to all of the tissues and high cl
earance. It is concluded that various strains of bacteria translocate from
the intestine to a similar degree after injury, but the tissues to which th
ey translocate and the rate at which they are killed are somewhat strain de
pendent.