Wool can be an appropriate medium for growing and transferring microorganis
ms under favorable temperature and humidity conditions. These microorganism
s can result in wool damage, skin irritation, or infections. A new and effe
ctive quaternary ammonium surfactant, N-dodecyl-N, N-dimethyl glycine cyste
amine hydrochloride (DABm), has recently been synthesized. DABM can react w
ith wool by means of its thiol group, either with cysteine-S-sulplionate re
sidues (Bunte salts) of sodium bisulphite pretreated wool or with the disul
fide bond of cystine wool, forming an asymmetrical disulfide bond. The anti
microbial activity of bisulphite-pretreated and untreated wool, both treate
d with DABM, is studied against gram-positive and gram-negative bacteria by
means of the protective antibacterial AATCC, test method 100-1989 and SEM
observation. Both wool samples show biocide activity against B. pumilus and
bacteriostatic activity against S. aureus. The absence of gram-positive an
d gram-negative bacteria attached to the wool surface fibers is observed by
SEM.