Determination of LSD in urine with high-performance liquid chromatography-mass spectrometry

A rapid, specific, and sensitive high-performance liquid chromatography/mas s spectrometry method has been developed for routine determination of lyser gic acid diethylamide (LSD) in urine. It includes sample purification by ex traction into an organic solvent and back-extraction to an acetate buffer, reversed-phase highperformance liquid chromatography, and detection with a single quadrupole mass spectrometer equipped with an atmospheric pressure i onization electrospray interface. Trideuterated LSD was used as internal st andard. The limit of detection was 0.02 ng/mL and the calibration curve was linear from 0.05 to 10 ng/mL. Within- and between-day coefficients of vari ation were 3.5% and 4.0% respectively and extraction recovery was 91%.