The binding of soybean agglutinin (SBA) to the intestinal epithelium of Atlantic salmon, Salmo salar and Rainbow trout, Oncorhynchus mykiss, fed highlevels of soybean meal
Lg. Buttle et al., The binding of soybean agglutinin (SBA) to the intestinal epithelium of Atlantic salmon, Salmo salar and Rainbow trout, Oncorhynchus mykiss, fed highlevels of soybean meal, VET IMMUNOL, 80(3-4), 2001, pp. 237-244
A lectin present in soya, soybean agglutinin (SBA), was identified in elect
rophoretic profiles and immunoblots of dehulled solvent-extracted soybean m
eal (DSSM), full-fat soybean meal (FFSM) and of aqueous extracts of feeds i
ncorporating them in their formulation. A quantitative estimation was made
of the proportion of SBA comprising the total protein in FFSM and a trial d
iet was prepared containing an amount of pure SBA similar to that in diets
incorporating high levels of the whole soya product. Fish fed with this die
t exhibited similar pathological disruption of the intestinal tract to that
observed in fish given a diet with a high level of DSSM (60% of the diet).
Furthermore, immuno-histochemistry revealed the binding of the SBA to the
enterocytes lining the intestinal villi both of fish fed a diet incorporati
ng pure SBA and those fed a diet containing a high-level of soya (60%). Our
results suggest that SBA binds in vivo to the intestinal epithelium of fis
h and has a contributory role in pathological changes associated with fish
feeds containing high levels of soybean proteins. (C) 2001 Elsevier Science
BN. All rights reserved.