Determination of the oxidative burst chemiluminescent response of avian and murine-derived macrophages versus corresponding cell lines in relation tostimulation with Salmonella serotypes

In contrast to mammalian systems, avian species lack a resident or harvesta ble, macrophage population in the abdominal exudate. Peritoneal macrophages in the chicken can be elicited if an inflammatory agent such as sephadex i s injected. This study examines the kinetics of different macrophage popula tions, derived by different methods of isolation and from different hosts, with respect to the elicited oxidative burst upon infection with host-adapt ed Salmonella serotypes. The nature of the oxidative burst elicited by murine and avian-derived and cell line macrophages was determined after stimulation with phorbol myrista te (PMA), zymosan A, and Salmonella serotypes. Both murine and chicken peri toneal macrophages, chicken blood monocytes and corresponding cell lines, J 774A.1 and HD-11, were unable to produce a detectable chemiluminescent (CL) response after interaction with Salmonella using the luminescent probe lum inol. However, both PMA and zymosan A induced a CL response in all cell typ es, with PMA eliciting a higher and earlier peak response (pkH) than zymosa n A. Lucigenin-enhanced CL in both murine and chicken macrophages was achie ved with PMA, zymosan A and Salmonella serotypes. In this case, zymosan A i nduced higher responses than PMA. In the peritoneal macrophages of both hos ts, there were no significant differences in the oxidative burst induced by the different Salmonella serotypes. However, the J774A.1 (murine) cells de monstrated significant differences, with S. enterica serotype Choleraesuis (S. choleraesuis and S. gallinarum producing the highest response. In the H D-11 (chicken) cells, S. choleraesuis and S. dublin elicited the higher CL. With both cell lines, S. abortusovis failed to induce an appreciable CL re sponse. In these experiments it was demonstrated that oxidative burst was not detec table in monocytes/macrophage populations using luminol, which suggests a l ink to die lack of a myeloperoxidase system in these cells. Lucigenin-enhan ced CL appeared independent from the myeloperoxidase system, indicating pro duction of another oxidative species compared with luminol. No discernable effect of host specificity with regard to Salmonella serotype and respectiv e host was seen in host-derived or cell line macrophages, and cell line mac rophages displayed altered functional characteristics with regard to oxidat ive burst in comparison with their primary counterparts. (C) 2001 Elsevier Science B.V. All rights reserved.