Eb. Naidoo et Jf. Van Staden, An improved technique for the determination of oxidised nitrogen in natural waters with a sequential injection analysis (SIA) system, WATER SA, 27(3), 2001, pp. 355-360
An SIA systems proposed for the determination of oxidised nitrogen (nitrate
+ nitrite as N) in natural waters. A cadmium reductor, made of cadmium gra
nules, closely packed in a glass column reduces the nitrate to nitrite. The
reduced nitrate and the nitrite present in the water samples is diazotised
in the SIA system with sulphanilamide and coupled with N - (1-napthyl) eth
ylene diammoniumdichloride to form a highly coloured azo dye which is detec
ted at 540 nm with a UV/Vis spectrophotometer. The proposed system is fully
computerised and is able to monitor total oxidised nitrogen as nitrite at
a frequency of 36 samples per hour with a standard deviation of < 1.2%, The
calibration curve is linear up to 5 mg/l with a detection limit of 0.01 mg
/l.