Astrovirus detection in wastewater samples

Procedures for the detection of astroviruses in wastewater samples have tee n developed and evaluated. Following these methodologies, we investigated t he occurrence of astroviruses in wastewater samples from three different se wage treatments plants located in Southern France and two in the Barcelona area. Some positive samples were genotyped by analysis of a fragment of the ORF 1a by restriction fragment length polymorphism (RFLP) with endonucleas e Dde I. The amplimers generated contain several sites for the Ddel restric tion endonuclease, being the number and location of sites different between strains.