Differentiation between non-virulent and virulent Burkholderia pseudomallei with monoclonal antibodies to the Ara(+) or Ara(-) biotypes

Citation
C. Thepthai et al., Differentiation between non-virulent and virulent Burkholderia pseudomallei with monoclonal antibodies to the Ara(+) or Ara(-) biotypes, AM J TROP M, 65(1), 2001, pp. 10-12
Citations number
18
Categorie Soggetti
Envirnomentale Medicine & Public Health","Medical Research General Topics
Journal title
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
ISSN journal
00029637 → ACNP
Volume
65
Issue
1
Year of publication
2001
Pages
10 - 12
Database
ISI
SICI code
0002-9637(200107)65:1<10:DBNAVB>2.0.ZU;2-S
Abstract
Burkholderia pseudomallei is the causative agent of melioidosis, a fatal tr opical infectious disease endemic in Southeast Asia. Environmental isolates of B. pseudomallei have two distinctive biotypes. Some soil isolates are a rabinose-assimilators (Ara(+) biotype) and are non-virulent in experimental animals. The others cannot assimilate arabinose (Ara(-) biotype) and are v irulent in experimental animals. The Ara(-) biotype is found in almost all B. pseudomallei clinical isolates. In the present study, a panel of eight m onoclonal antibodies that agglutinate the bacteria were produced and tested . The first group, Bps-D2. -D3, -D5, -L1, and -L2 agglutinated 100% of Aral clinical and soil isolates of B. pseudomallei. Another group Bps-A I, -A2, and -D1 agglutinated 92.9% and 90.9% of Ara(-) clinical and soil isolates. respectively. This panel of monoclonal antibodies may be useful for rapid differentiation between non-virulent Ara(+) and virulent Ara(-) B. pseudoma llei.