C. Thepthai et al., Differentiation between non-virulent and virulent Burkholderia pseudomallei with monoclonal antibodies to the Ara(+) or Ara(-) biotypes, AM J TROP M, 65(1), 2001, pp. 10-12
Citations number
18
Categorie Soggetti
Envirnomentale Medicine & Public Health","Medical Research General Topics
Burkholderia pseudomallei is the causative agent of melioidosis, a fatal tr
opical infectious disease endemic in Southeast Asia. Environmental isolates
of B. pseudomallei have two distinctive biotypes. Some soil isolates are a
rabinose-assimilators (Ara(+) biotype) and are non-virulent in experimental
animals. The others cannot assimilate arabinose (Ara(-) biotype) and are v
irulent in experimental animals. The Ara(-) biotype is found in almost all
B. pseudomallei clinical isolates. In the present study, a panel of eight m
onoclonal antibodies that agglutinate the bacteria were produced and tested
. The first group, Bps-D2. -D3, -D5, -L1, and -L2 agglutinated 100% of Aral
clinical and soil isolates of B. pseudomallei. Another group Bps-A I, -A2,
and -D1 agglutinated 92.9% and 90.9% of Ara(-) clinical and soil isolates.
respectively. This panel of monoclonal antibodies may be useful for rapid
differentiation between non-virulent Ara(+) and virulent Ara(-) B. pseudoma
llei.